3HYR

Structural Insight into G Protein Coupling and Regulation of Fe2+ Membrane Transport

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-01
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	C 1 2 1
Unit cell lengths	146.120, 84.440, 66.240
Unit cell angles	90.00, 108.46, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.22817
Rwork	0.225
R-free	0.28718
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	1.486
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.5.0063)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.170	2.320
High resolution limit [Å]	2.200	2.200
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	30% PEG 400, 100mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K