3HYO
Crystal structure of pyridoxal kinase from Lactobacillus plantarum in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.594, 69.761, 132.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.060 - 1.850 |
| R-factor | 0.18208 |
| Rwork | 0.180 |
| R-free | 0.22219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h74 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.478 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.060 | 1.897 |
| High resolution limit [Å] | 1.850 | 1.849 |
| Rmerge | 0.050 | 0.313 |
| Number of reflections | 24694 | |
| <I/σ(I)> | 13.5 | 6.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M MgCl2, 0.1M HEPES pH 6.5, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
