3HYN

Crystal structure of a putative signal transduction protein (eubrec_0645) from eubacterium rectale atcc 33656 at 1.20 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-13
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	34.797, 60.962, 76.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.461 - 1.200
R-factor	0.118
Rwork	0.117
R-free	0.13900
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.533
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.461	32.461	1.240
High resolution limit [Å]	1.200	2.580	1.200
Rmerge	0.076	0.056	0.374
Number of reflections	51559	5483	4529
<I/σ(I)>	9.1	23.6	2.4
Completeness [%]	99.3	99	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	40.0000% MPD, 0.1M Bicine pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K