3HYM

Insights into Anaphase Promoting Complex TPR subdomain assembly from a CDC26-APC6 structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Wavelength(s)	0.97926
Spacegroup name	P 63
Unit cell lengths	301.897, 301.897, 80.168
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.400 - 2.800
R-factor	0.1894
Rwork	0.188
R-free	0.21970
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.127	0.475
Number of reflections	197947
<I/σ(I)>	19	3.6
Completeness [%]	99.7	99.2
Redundancy	5	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	297	1:1 mix with well buffer: 0.2M MgCl2, 7% isopropanol, 0.1 M Tris-Cl pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K