3HYD
LVEALYL peptide derived from human insulin chain B, residues 11-17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97645 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 49.478, 4.838, 19.442 |
| Unit cell angles | 90.00, 96.65, 90.00 |
Refinement procedure
| Resolution | 16.140 - 1.000 |
| R-factor | 0.149 |
| Rwork | 0.146 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | an idealized beta strand |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.790 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.080 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.184 | 0.361 |
| Number of reflections | 2647 | |
| <I/σ(I)> | 7.152 | 3.26 |
| Completeness [%] | 95.1 | 84.7 |
| Redundancy | 5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20% MPD, 0.1M sodium citrate pH 5.5, vapor diffusion, hanging drop, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20% MPD, 0.1M sodium citrate pH 5.5, vapor diffusion, hanging drop, temperature 298K |
