3HYB

Crystal structure of RbcX from Anabaena, crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	74.990, 74.990, 123.841
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.247
Rwork	0.246
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2peo
RMSD bond length	0.014
RMSD bond angle	1.364
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.510	64.960	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge	0.059	0.028	0.301
Total number of observations		3087	10479
Number of reflections	18396
<I/σ(I)>	8.468	19.5	2.5
Completeness [%]	99.5	99.1	97.7
Redundancy	5.1	4.7	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	1 M (NH4)2SO4, 0.1 M Bis-Tris-HCl pH 5.5, 1 % PEG-3350, vapor diffusion, sitting drop, temperature 291K