3HXR

Nucleoporin Nup120 from S.cerevisiae (aa 1-757)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	114.600, 153.690, 52.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.090 - 3.000
R-factor	0.247
Rwork	0.244
R-free	0.29900
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.915
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	19288
<I/σ(I)>	20.4
Completeness [%]	99.8	99.6
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	15% PEG3350, 0.2M KSCN, 0.1M Tris-HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K