3HXL
Crystal Structure of the sheath tail protein (DSY3957) from Desulfitobacterium hafniense, Northeast Structural Genomics Consortium Target DhR18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.382, 71.958, 108.831 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.900 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB (then SOLVE/RESOLVE) |
| Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 0.206 |
| Number of reflections | 80185 | |
| <I/σ(I)> | 22.4 | 4.4 |
| Completeness [%] | 97.5 | 89.4 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch, under oil | 7.5 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 100 mM Tris-HCl (pH 7.5), 10% PEG3350, and 20% ethylene glycol., microbatch, under oil, temperature 277K |
