3HXK
Crystal Structure of a sugar hydrolase (YeeB) from Lactococcus lactis, Northeast Structural Genomics Consortium Target KR108
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.391, 150.425, 218.859 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 3.200 |
| R-factor | 0.257 |
| Rwork | 0.256 |
| R-free | 0.29600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB (then SOLVE/RESOLVE) |
| Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.088 | 0.172 |
| Number of reflections | 36064 | |
| <I/σ(I)> | 17.4 | 3.2 |
| Completeness [%] | 92.6 | 74.5 |
| Redundancy | 5.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch, under oil | 9.1 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1 M TRIS (pH 9.1), 29% PEG400, and 0.1M (Mg)2SO4. , microbatch, under oil, temperature 291K |
