3HWU

Crystal structure of Putative DNA-binding protein (YP_299413.1) from Ralstonia eutrophA JMP134 at 1.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97929,0.97918
Spacegroup name	H 3
Unit cell lengths	53.897, 53.897, 111.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.948 - 1.300
R-factor	0.121
Rwork	0.119
R-free	0.14000
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.644
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.948	26.948	1.350
High resolution limit [Å]	1.300	2.800	1.300
Rmerge	0.028	0.017	0.258
Number of reflections	29085	5755	4046
<I/σ(I)>	13.78	34.1	2.4
Completeness [%]	91.6	96.6	64
Redundancy	2.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	277	0.2000M NaCl, 20.0000% PEG-1000, 0.1M Na,K-Phosphate pH 6.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K