3HWT

Ternary complex of DNA polymerase lambda bound to a two nucleotide gapped DNA substrate with a scrunched dA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-22
Detector	MAR CCD 165 mm
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.023, 68.328, 137.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.950
Rwork	0.228
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsn
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.089	0.070	0.399
Number of reflections	38019
<I/σ(I)>	10.283
Completeness [%]	96.0	85.8	91.2
Redundancy	4.1	6.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	19% 2-propanol, ).1 M Na Cacodylate, pH 5.5, Na Citrate 0.2M, VAPOR DIFFUSION, HANGING DROP, temperature 277K