3HVZ
Crystal Structure of the TGS domain of the CLOLEP_03100 protein from Clostridium leptum, Northeast Structural Genomics Consortium Target QlR13A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-28 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97945 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.765, 110.396, 107.957 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.700 - 2.200 |
R-factor | 0.225 |
Rwork | 0.224 |
R-free | 0.25400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.082 | 0.483 |
Number of reflections | 62970 | |
<I/σ(I)> | 25.4 | 2.9 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 7.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 100mM HEPES (pH 7.5) and 500mM magnesium Formate dihydrate, microbatch, under oil, temperature 277K |