3HVW

Crystal Structure of the GGDEF domain of the PA2567 protein from Pseudomonas aeruginosa, Northeast Structural Genomics Consortium Target PaR365C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97903
Spacegroup name	P 21 21 21
Unit cell lengths	49.374, 53.757, 76.792
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.180 - 1.700
R-factor	0.202
Rwork	0.201
R-free	0.21900
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB (then SOLVE/RESOLVE)
Refinement software	CNS (1.2 & XtalView)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.028	0.078
Number of reflections	43035
<I/σ(I)>	49.2	23.7
Completeness [%]	98.9	100
Redundancy	7.4	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch, under oil	8	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 100mM TRIS (pH 8) and 800mM Ammonium Sulfate, microbatch, under oil, temperature 277K