3HVD
The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0712 |
| Spacegroup name | P 1 |
| Unit cell lengths | 125.602, 125.670, 125.823 |
| Unit cell angles | 106.64, 110.82, 110.98 |
Refinement procedure
| Resolution | 47.274 - 3.209 |
| R-factor | 0.2118 |
| Rwork | 0.210 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tzo |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 2009_02_15_2320_3)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.275 | 3.245 |
| High resolution limit [Å] | 3.209 | 3.209 |
| Rmerge | 0.125 | 0.595 |
| Number of reflections | 87387 | |
| <I/σ(I)> | 10.78 | 2.1 |
| Completeness [%] | 98.1 | 90.28 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 74 mM sodium acetate, 7 mM Tris, 0.62 M NaCl, 37 mM tetrabutylammonium bromide, 7% ethanol, 0.07% n-dodecyl-D-maltopyranoside, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
