3HVA
Crystal structure of FimX GGDEF domain from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.179, 94.111, 45.504 |
| Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
| Resolution | 27.118 - 2.042 |
| R-factor | 0.2143 |
| Rwork | 0.213 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w25 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.723 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.174 |
| Number of reflections | 17415 | |
| <I/σ(I)> | 18.7 | 6.4 |
| Completeness [%] | 93.6 | 76.1 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG MME 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
