3HV6
Human p38 MAP Kinase in Complex with RL39
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-11 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54170 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.410, 74.660, 78.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.503 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.033 | 0.217 |
| Number of reflections | 28964 | 3966 |
| <I/σ(I)> | 30.39 | 7.7 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.66 | 4.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
