3HV4
Human p38 MAP Kinase in Complex with RL51
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-05-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.977032 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.640, 74.060, 72.000 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.600 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyj |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.598 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.111 | 0.499 |
| Number of reflections | 22870 | 2438 |
| <I/σ(I)> | 19.13 | 5.4 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 3.5 | 3.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
