3HUT
Crystal structure of a putative branched-chain amino acid ABC transporter from Rhodospirillum rubrum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.341, 92.706, 44.432 |
| Unit cell angles | 90.00, 115.10, 90.00 |
Refinement procedure
| Resolution | 40.240 - 1.930 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 23896 | |
| <I/σ(I)> | 7.4 | 5.5 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 7.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 200mM Magnesium Acetate tetrahydrate + 100mM Sodium Cacodylate pH 6.5 + 20% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
