3HUE
Structure of the S. pombe Nbs1 FHA-BRCT1-BRCT2 domains
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796, 0.9797 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.671, 204.538, 62.897 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.23826 |
| Rwork | 0.236 |
| R-free | 0.28518 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.426 |
| Number of reflections | 16723 | |
| <I/σ(I)> | 13.9 | 2.75 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 281 | 1.2-1.4M potassium formate, 100mM sodium acetate pH 4.6, 5% w/v polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
