3HU7
Structural characterization and binding studies of a plant pathogenesis related protein heamanthin from haemanthus multiflorus reveal its dual inhibitory effects against xylanase and alpha-amylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54312 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.803, 65.400, 49.435 |
| Unit cell angles | 90.00, 102.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.000 |
| R-factor | 0.15372 |
| Rwork | 0.152 |
| R-free | 0.18632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hvm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.811 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 16787 | |
| Completeness [%] | 97.5 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.1M ammonium sulphate, 20mM phosphate buffer, 0.1M sodium acetate, 20% PEG 6000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
