3HTZ

Crystal Structure of the catalytic fragment of alanyl-tRNA synthetase in complex with L-serine: Re-refined

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 41 21 2
Unit cell lengths	74.091, 74.091, 173.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.050 - 2.500
R-factor	0.25327
Rwork	0.250
R-free	0.31531
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1yfs
RMSD bond length	0.003
RMSD bond angle	0.763
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.480	2.480
Number of reflections	17674
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	298	PEG 5000 mme, NaCl, Tris-Cl, Tris-base, L-serine, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K