3HTS
HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.690, 54.900, 41.060 |
| Unit cell angles | 90.00, 122.44, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| Rwork | 0.208 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hts |
| RMSD bond length | 0.001 * |
| RMSD bond angle | 1.480 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.035 | 0.095 |
| Total number of observations | 34273 * | |
| Number of reflections | 10653 | 750 * |
| <I/σ(I)> | 10.3 | 7.4 |
| Completeness [%] | 89.4 | 86 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.91 (mM) | |
| 2 | 1 | drop | DNA | 1.47 (mM) | |
| 3 | 1 | reservoir | isopropanol | 15 (%(v/v)) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | |
| 5 | 1 | reservoir | 20 (mM) | ||
| 6 | 1 | reservoir | potassium glutamate | 100 (mM) |
