3HTR
Crystal Structure of PRC-barrel Domain Protein from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.644, 63.061, 61.866 |
| Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
| Resolution | 28.010 - 2.060 |
| R-factor | 0.197 |
| Rwork | 0.193 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.379 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.4_58)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.010 | 2.100 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 11619 | |
| <I/σ(I)> | 7.4 | 4.1 |
| Completeness [%] | 95.3 | 78 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1 M Bis-tris-Propane pH 7.0, 1.3 M di-ammonium tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
