3HTK
Crystal structure of Mms21 and Smc5 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2006-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97899 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.465, 91.574, 249.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.310 |
| R-factor | 0.257 |
| Rwork | 0.256 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.266 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 21519 | |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 88.0 | 77.3 |
| Redundancy | 4.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 18% PEG 3350, 40mM CaCl2, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 18% PEG 3350, 40mM CaCl2, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
