3HTE
Crystal structure of nucleotide-free hexameric ClpX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.325, 199.917, 202.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.074 - 4.026 |
| R-factor | 0.279 |
| Rwork | 0.278 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.520 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 8.600 | 4.000 |
| Rmerge | 0.054 | 0.029 | 0.488 |
| Number of reflections | 21881 | ||
| <I/σ(I)> | 55 | ||
| Completeness [%] | 99.9 | 99.4 | 100 |
| Redundancy | 14.4 | 13.2 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 75 mM Sodium acetate, 1.9 M ammonium sulfate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
