3HTA

Crystal structure of multidrug binding protein EbrR complexed with imidazole

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-09
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	76.471, 77.922, 203.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.700 - 2.300
R-factor	0.2524
Rwork	0.252
R-free	0.29220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MODEL FORM MAD DATA OF EBRR
RMSD bond length	0.006
RMSD bond angle	1.260
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	67.700
High resolution limit [Å]	2.300
Rmerge	0.030
Number of reflections	54199
<I/σ(I)>	48.3
Completeness [%]	99.6
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	295	30% PEG 1000, 0.1 M Calcium chloride, 0.1 M Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K