3HSP
Ternary structure of neuronal nitric oxide synthase with NHA and NO bound(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.820, 110.560, 164.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.820 - 2.200 |
| R-factor | 0.167 |
| Rwork | 0.164 |
| R-free | 0.21700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB CODE 1LZX |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 | 0.505 |
| Number of reflections | 48552 | |
| <I/σ(I)> | 16.9 | 3.5 |
| Completeness [%] | 98.9 | 97.6 |
| Redundancy | 4.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 278 | PEG3350, MES, ammonium acetate SDS, GSH, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
