3HSO
Ternary structure of neuronal nitric oxide synthase with NHA and NO bound(1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.830, 110.440, 164.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.150 - 2.020 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.22400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB CODE 1LZX |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.424 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.037 | 0.256 |
| Number of reflections | 59511 | |
| <I/σ(I)> | 21.4 | 3.4 |
| Completeness [%] | 94.2 | 91 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 278 | PEG3350, MES, ammonium acetate, SDS, GSH, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
