3HSK
Crystal Structure of Aspartate Semialdehyde Dehydrogenase with NADP from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03319 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.412, 152.180, 97.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.710 - 2.200 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ys4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.115 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.710 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.467 |
| Number of reflections | 32719 | |
| <I/σ(I)> | 20.167 | |
| Completeness [%] | 91.5 | 60.6 |
| Redundancy | 5.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 400, 0.1 M HEPES pH 6.5, 0.1 M Magnesium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
