3HSH
Crystal structure of human collagen XVIII trimerization domain (Tetragonal crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-24 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.106 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 71.745, 71.745, 134.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.524 - 1.800 |
| R-factor | 0.1795 |
| Rwork | 0.175 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.090 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.440 |
| Number of reflections | 33455 | |
| <I/σ(I)> | 24.5 | 5.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 1.65M ammonium sulfate, 0.1M citric acid, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
