3HRZ
Cobra Venom Factor (CVF) in complex with human factor B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9834 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 128.890, 283.390, 134.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.487 - 2.200 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2OK5 and 2I07 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.093 | 0.602 |
| Total number of observations | 67190 | |
| Number of reflections | 123939 | |
| <I/σ(I)> | 11.8 | 2.6 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | PEG 3350, PEG 400, Na/K Phosphate, Bis-Tris propane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
