Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-10-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.488, 139.368, 156.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.680 - 1.850 |
| Rwork | 0.177 |
| R-free | 0.20600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.037 | 0.349 |
| Number of reflections | 139053 | |
| <I/σ(I)> | 32.8 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Reservoir solution containing 20% (v/v) PEG MME2000, 0.05M KH2PO4, 5% (v/v) non-detergent sulfobetain (NDSB) 201, and 20% (v/v) glycerol were mixed with protein solution in a 1:1, and equilibrated against the reservoir solution. , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
