3HRI
Histidyl-tRNA synthetase (apo) from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97923 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 148.610, 98.380, 251.969 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.850 |
| R-factor | 0.274 |
| Rwork | 0.272 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | T. cruzi Histidyl-tRNA synthetase |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.173 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 128.322 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 99984 |
| <I/σ(I)> | 6 |
| Completeness [%] | 100.0 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 2 M Lithium Sulfate, 0.1 M HEPES pH 7.3, 1mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
