3HR0

Crystal structure of Homo sapiens Conserved Oligomeric Golgi subunit 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0809
Spacegroup name	P 32 2 1
Unit cell lengths	87.114, 87.114, 214.773
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.590 - 1.900
R-factor	0.196
Rwork	0.194
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.191
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.077	0.061	0.357
Number of reflections	74854
<I/σ(I)>	26.485
Completeness [%]	99.3	98.5	98.2
Redundancy	5.4	6.6	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	5% PEG-4000, 10 mM ammonium nitrate, 2.5% glycerol, 12 mM DTT, 10 mM Tris, 150 mM potassium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	5% PEG-4000, 10 mM ammonium nitrate, 2.5% glycerol, 12 mM DTT, 10 mM Tris, 150 mM potassium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K