3HQF

Crystal structure of restriction endonuclease EcoRII N-terminal effector-binding domain in complex with cognate DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-11
Detector	MAR CCD 165 mm
Wavelength(s)	0.8080
Spacegroup name	P 43 21 2
Unit cell lengths	43.156, 43.156, 253.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.500 - 2.510
R-factor	0.191
Rwork	0.187
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1na6
RMSD bond length	0.016
RMSD bond angle	1.916
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.19)
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.500	2.640
High resolution limit [Å]	2.504	2.500
Rmerge	0.068	0.079
Total number of observations		7469
Number of reflections	9055
<I/σ(I)>	6.856	7
Completeness [%]	99.1	94.3
Redundancy	6.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	1M sodium acetate, 0.2M lithium acetate, 10% glycerol, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K