3HQE
Crystal Structure of the decamer CGGGCGCCCG forming a Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.004, 22.813, 40.708 |
| Unit cell angles | 90.00, 116.75, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.940 |
| R-factor | 0.2166 |
| Rwork | 0.215 |
| R-free | 0.23946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1P4Y |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.158 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.118 | 0.458 |
| Number of reflections | 1239 | |
| <I/σ(I)> | 2.9 | 1 |
| Completeness [%] | 97.9 | 96.5 |
| Redundancy | 3.51 | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Drop contains 1mM DNA, 50mM buffer, 100mM MgCl2, 1mM spermine, 50% MPD in reservior , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
