3HQ2
BsuCP Crystal Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2834 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 88.957, 149.002, 217.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.370 - 2.900 |
| R-factor | 0.1879 |
| Rwork | 0.188 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.370 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.188 | 0.302 |
| Number of reflections | 31847 | |
| Completeness [%] | 96.6 | 92.5 |
| Redundancy | 4.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | 0.2 M NH4F, 32% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
