3HOR
Structure of the actin-binding domain of human filamin A (reduced)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.672, 72.290, 155.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.092 - 2.700 |
| R-factor | 0.2284 |
| Rwork | 0.225 |
| R-free | 0.29390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hop |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.097 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.100 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.120 | 0.590 |
| Total number of observations | 72824 | |
| Number of reflections | 17832 | |
| <I/σ(I)> | 11.4 | 2 |
| Completeness [%] | 96.1 | 84 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, 5mM DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
