3HON
Crystal Structure of Human Collagen XVIII Trimerization Domain (cubic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-24 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.106 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 94.876, 94.876, 94.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.730 - 3.000 |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.074 | 0.655 |
| Number of reflections | 1634 | |
| <I/σ(I)> | 25.9 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.6 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.25M MgCl2, 0.1M BisTris, 18-22% (w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
