3HOL
The Structure of Intact Ap-TbpB (N and C lobes)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.360, 96.807, 143.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.600 - 1.980 |
| R-factor | 0.18744 |
| Rwork | 0.185 |
| R-free | 0.23142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hoe |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.462 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (CCP4) |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.600 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.331 | |
| Number of reflections | 33311 | |
| <I/σ(I)> | 24.3 | 4.9 |
| Completeness [%] | 98.6 | 86.7 |
| Redundancy | 6.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 20% PEG3350, 0.1M Tris pH 8.0, 150 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
