3HOE
Crystal Structure of Surface Lipoprotein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98057, 0.98088, 0.97007 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.473, 43.431, 100.397 |
| Unit cell angles | 90.00, 118.16, 90.00 |
Refinement procedure
| Resolution | 35.308 - 2.303 |
| R-factor | 0.1979 |
| Rwork | 0.194 |
| R-free | 0.26120 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.310 |
| High resolution limit [Å] | 2.303 |
| Number of reflections | 42372 |
| <I/σ(I)> | 29.6 |
| Completeness [%] | 95.0 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 20% PEG3350, 0.1M Tris pH 8.0, 150 mM NaCl , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
