3HO4
Crystal structure of Hedgehog-interacting protein (HHIP)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97940, 0.97955, 0.91176 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.042, 101.042, 304.923 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| R-factor | 0.21926 |
| Rwork | 0.217 |
| R-free | 0.25237 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.203 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 33657 | |
| <I/σ(I)> | 21.2 | 2.9 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 292 | Protein samples (0.3-2 ul) were dehydrated over 500 ul of reservoir solution of 20 mM Hepes pH 7.2 and 3 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
