3HNW

Crystal Structure of a Basic Coiled-Coil Protein of Unknown Function from Eubacterium eligens ATCC 27750

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-03
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	231.182, 53.837, 40.917
Unit cell angles	90.00, 99.83, 90.00

Refinement procedure

Resolution	27.849 - 2.196
R-factor	0.215
Rwork	0.213
R-free	0.24430
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.229
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.4_58))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.850	2.240
High resolution limit [Å]	2.196	2.200
Rmerge		0.392
Number of reflections	25331
<I/σ(I)>	13	2.8
Completeness [%]	99.3	97.9
Redundancy	3.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.3M Na Iodide, 10% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K