3HNL
Crystal structure of the Cu-induced dimer of the engineered cyt cb562 variant RIDC-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-28 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.165, 87.045, 80.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.200 - 2.200 |
| R-factor | 0.208 |
| Rwork | 0.204 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qla |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.200 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.168 | 0.480 |
| Number of reflections | 12016 | |
| <I/σ(I)> | 6.5 | 2 |
| Completeness [%] | 98.7 | 96.5 |
| Redundancy | 3.12 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES, 200 mM sodium chloride, 25% PEG 3350, 10.7 mM copper sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
