3HNJ
Crystal structure of the Zn-induced tetramer of the engineered cyt cb562 variant RIDC-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.448, 65.316, 70.773 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 30.700 - 2.000 |
| R-factor | 0.22788 |
| Rwork | 0.224 |
| R-free | 0.27159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qla |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.185 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.700 | 68.360 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.044 | 0.036 | 0.176 |
| <I/σ(I)> | 11.022 | 18.5 | 2.7 |
| Completeness [%] | 92.5 | 69.4 | 92.4 |
| Redundancy | 2.6 | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM BIS-TRIS, 25% PEG 3350, 3.4 mM zinc chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
