3HMU
Crystal structure of a class III aminotransferase from Silicibacter pomeroyi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97958 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.246, 113.605, 130.985 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.19300 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.523 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.134 | 0.310 |
Number of reflections | 56398 | |
<I/σ(I)> | 9.884 | 2.74 |
Completeness [%] | 99.3 | 94.5 |
Redundancy | 8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM sodium cacodylate pH 6.5, 30% PEG 8K, 200mM ammonium sulfate, vapor diffusion, temperature 294K |