3HMB

Mutant endolysin from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-02-07
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	I 2 2 2
Unit cell lengths	62.670, 152.760, 188.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.700
Rwork	0.213
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3HMA
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.200
Number of reflections	25390
<I/σ(I)>	10
Completeness [%]	99.9	100
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1