3HM9

Crystal structure of T. thermophilus Argonaute complexed with DNA guide strand and 19-nt RNA target strand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-03-17
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	111.916, 111.916, 175.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.300
R-factor	0.239
Rwork	0.237
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.398
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.380
High resolution limit [Å]	3.300	3.300
Rmerge	0.060	0.444
Number of reflections	17434
<I/σ(I)>	26.3	2.6
Completeness [%]	99.5	100
Redundancy	7.3	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	308	50 mM MgCl2, 1.0 M Na tartrate, 50 mM Tris.HCl pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 308 K