3HM9
Crystal structure of T. thermophilus Argonaute complexed with DNA guide strand and 19-nt RNA target strand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-03-17 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 111.916, 111.916, 175.551 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.300 |
R-factor | 0.239 |
Rwork | 0.237 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.398 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.380 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.060 | 0.444 |
Number of reflections | 17434 | |
<I/σ(I)> | 26.3 | 2.6 |
Completeness [%] | 99.5 | 100 |
Redundancy | 7.3 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 308 | 50 mM MgCl2, 1.0 M Na tartrate, 50 mM Tris.HCl pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 308 K |