3HLY
Crystal Structure of the Flavodoxin-like domain from Synechococcus sp Q5MZP6_SYNP6 protein. Northeast Structural Genomics Consortium Target SnR135d.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-29 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9786, 0.97917, 0.91837 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.270, 97.899, 118.076 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.937 - 2.403 |
| R-factor | 0.2259 |
| Rwork | 0.225 |
| R-free | 0.24990 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.329 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.053 | 0.168 |
| Number of reflections | 32571 | |
| <I/σ(I)> | 39.5 | 11.5 |
| Completeness [%] | 97.9 | 100 |
| Redundancy | 5.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under paraffin oil | 6 | 293 | 20% PEG 8000, 0.1M Ca acetate, 0.1M MES, pH 6.0, Microbatch under paraffin oil , temperature 293K |
