3HLU
Crystal Structure of Uncharacterized Protein Conserved in Bacteria DUF2179 from Eubacterium ventriosum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.588, 63.392, 82.197 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.103 - 2.650 |
| R-factor | 0.227 |
| Rwork | 0.226 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.295 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.108 | 0.698 |
| Number of reflections | 10088 | |
| <I/σ(I)> | 11.7 | 2.6 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 7.6 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M NaCl, 0.1 M HEPES pH 7.5, 30% (v/v) PEG-400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
